Cotyledonary node pre-wounding with a micro-brush increased frequency of Agrobacterium-mediated transformation in soybean
نویسندگان
چکیده
منابع مشابه
Optimization of Agrobacterium mediated genetic transformation of cotyledonary node explants of Vigna radiata
A reproducible and highly efficient protocol for genetic transformation mediated by Agrobacterium has been established for greengram (Vigna radiata L. Wilczek). Double cotyledonary node (DCN) explants were inoculated with Agrobacterium tumefaciens strain LBA 4404 harboring a binary vector pCAMBIA 2301 containing neomycin phosphotransferase (npt II) gene as selectable marker, β-glucuronidase (GU...
متن کاملOptimization of Agrobacterium-Mediated Transformation in Soybean
High transformation efficiency is a prerequisite for study of gene function and molecular breeding. Agrobacterium tumefaciens-mediated transformation is a preferred method in many plants. However, the transformation efficiency in soybean is still low. The objective of this study is to optimize Agrobacterium-mediated transformation in soybean by improving the infection efficiency of Agrobacteriu...
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Lactuca sativa is widely used as a leafy vegetable belonging to the Asteraceae family. Cotyledonary leaves of in vitro lettuce seedlings were transformed by plasmids containing the GCHI gene. Also the effects of number of days of pre-cultivation and co-cultivation of explants were examined on the transformation frequency. After co-cultivation, the explants were transferred onto the regeneration...
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Revolution in plant biotechnology can be categorized into in vitro culture and genetic transformation. Plant regeneration was successfully achieved in 1950’s while production of transgenic plant was accomplished in 80’s. For production of transformants, in vitro culturing strategies are prerequisite. Soybean has been cultured through organogenesis and embryogenesis but still it is considered re...
متن کاملOptimized Selection and Regeneration Conditions for Agrobacterium-mediated Transformation of Chickpea Cotyledonary Nodes
An efficient selection system and Agrobacterium mediated transformation for chickpea (Cicer arietinum L.) cotyledonary nodes were investigated. Effect of selective agents and antibiotics on multiple shoot and root induction of cotyledonary nodes and effects of mechanical injury and vacuum infiltration on transformation efficiency were evaluated. Selective agents and antibiotics were applied to ...
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ژورنال
عنوان ژورنال: Plant Biotechnology
سال: 2010
ISSN: 1347-6114,1342-4580
DOI: 10.5511/plantbiotechnology.27.217